Keele Data Repository

Forsyth, Victor, Ahmadi, Delaram, Thompson, Katherine, Garcia Sakai, Victoria, Schweins, Ralf, Moulin, Martine, Haertlein, Michael, Strohmeier, Gernot. A, Pichler, Harald, Barlow, David J, Lawrence, M. Jayne and Foglia, Fabrizia (2022) Lipid dynamics in raft domains and effect of an oxidized bilayer component on their formation.

Lipid oxidation is linked to many adverse health effects. In this proposal we will use QENS in combination with contrast variation as a non-invasive method to detect and characterize the presence and size of microdomains formed in vesicles of biologically relevant lipids as a function of oxidized lipid levels and temperature. The domain forming lipid mixture system to be used in the present study will be composed of a saturated phospholipid, DPPC, an unsaturated phospholipid, POPC, and cholesterol (Chol) where a portion of the POPC is systematically replaced with the oxidized version of POPC, POnPC.